Formation of hydroxyapatite coatings with addition of chitosan from aqueous solutions by thermal substrate method | Журнал інженерних наук Журнал инженерных наук Journal Of Engineering Sciences

Formation of hydroxyapatite coatings with addition of chitosan from aqueous solutions by thermal substrate method

Author(s): Yanovska G. O.1, Bolshanina S. B.1*, Kuznetsov V. M.2

1Sumy State University, 2 Rymskogo-Korsakova St., 40007, Sumy, Ukraine
2Institute of Applied Chemistry of the National Academy of Sciences of Ukraine, 3 Rymskogo-Korsakova St., 40007, Sumy, Ukraine

*Corresponding Author’s Address:

Issue: Volume 4; Issue 2 (2017)

Paper receive: September 27, 2017
The final version of the paper received: December 1, 2017
Paper accepted online: December 3, 2017

Yanovska G. O. Formation of hydroxyapatite coatings with addition of chitosan from aqueous solutions by thermal substrate method / G. O. Yanovska, S. B. Bolshanina, V. M. Kuznetsov  // Journal of Engineering Sciences. —  Sumy : Sumy State University, 2017. — Volume 4, Issue 2. — P. F1-F4.

DOI: 10.21272/jes.2017.4(2).f1

Research Area: Material Sciences

Abstract: The aim of this investigation was obtaining of biocompatible coatings for medical implants based on biopolymer chitosan and hydroxyapatite, which is the main mineral component of bone tissue. Coatings were obtained by thermal substrate method, because it allows low temperature deposition and provides possibility to incorporate into coating structure biomolecules, unstable at high temperatures. As a way of chitosan incorporation into coating composition co-precipitation method was proposed. It allows obtaining uniform coatings with required composition and morphology. The obtained coatings were investigated by using of XRD, SEM with EDS, adhesion was tested by test-tape method. It was assigned that chitosan addition decreased hydroxyapatite crystallinity, so the range of concentrations from 0.01 to 0.1 g/L was chosen at pH = 6.5. It was determined that variation of chitosan concentrations in the initial solution influenced on morphology and structure of hydroxyapatite coatings as well as on the antibacterial properties and the use in orthopedics and dentistry. The best characteristics were obtained for hydroxyapatite-chitosan coatings deposited from solution with chitosan concentration 0.025 g/L.

Keywords:  hydroxyapatite, chitosan, coating, deposition, thermal substrate method.


  1. Wang, M. (2003). Developing bioactive composite materials for tissue replacement. Biomaterials, Vol. 24, 2133–2151.
  2. Elliott, J. C. (1994). Structure and chemistry of the apatites and other calcium orthophosphates. Elsevier. London, pp. 111–127.
  3. Vande V. P. J., Matthew, H. W. T., et al. (2002). Evalution of the biocompatibility of chitosan scaffold. J. Biomed. Mater. Res., Vol. 59, 585–90.
  4. Eugene, K., & Lee, Y. L. (2003). Implantable application of chitin and chitosan. Biomaterials, Vol. 24, 2339–2349.
  5. Di Martino, A., Sittinger, M., & Risbud, M. V. (2005). Chitosan: A versatile biopolymer for orthopedic tissue-engineering. Biomaterials, Vol. 26, 5983–5990.
  6. Je, J., & Kim, S. (2005). Water soluble chitosan derivatives as a BACE1 inhibitor. Bioorg. Med. Chem., Vol. 13, 6551–6555.
  7. Jeon, Y., Shahidi, F., & Kim, S. (2000). Preparation of chitin and chitosan oligomers and their applications in physiological functional foods. Food Rev. Int., Vol. 16, 159–176.
  8. Okuyama, K., Noguchi, K, et al. (1999). Structural study of anhydrous tendon chitosan obtained via chitosan/acetic acid complex. Int. J. Biol. Macromol., Vol. 26, 285–293.
  9. Ogawa, K., Hirano, S., et al. (1984). A new polymorph of chitosan. Macromolecules, Vol. 17, 973–975.
  10. Lee, Y. L., Khor, E., & Ling, C. E. (1999). Effects of dry heat and saturated steam on the physical properties of chitosan. J. Biomed. Mater. Res., Appl. Biomater., Vol. 48, 111–116.
  11. Yamaguchi, K., Tokuchi, H., et al. (2001). Preparation and microstructure analysis of chitosan/hydroxyapatite nanocomposites. J. Biomed. Mater. Res., Vol. 55, 20–27.
  12. Venkatesan, J., & Kim, S.-K. (2010). Review. Chitosan composites for bone tissue engineering – An overview. Mar. Drugs, Vol. 8, 2252–2266.
  13. Ding, S. (2007). Biodegradation behavior of chitosan/calcium phosphate composites. J. Non-Cryst. Solids, Vol. 353, 2367–2373.
  14. Li, Q., Chen, Z., et al. (2006). Biomimetic synthesis of the composites of hydroxyapatite and chitosan–phosphorylated chitosan polyelectrolyte complex. Mater. Lett., Vol. 60, 3533–3536.
  15. Verma, D., Katti, K., & Katti, D. (2008). Effect of biopolymers on structure of hydroxyapatite and interfacial interactions in biomimetically synthesized hydroxyapatite/biopolymer nanocomposites. Ann. Biomed. Eng. Vol. 36, 1024–1032.
  16. Davidenko, N., Carrodeguas, R., et al. (2010). Chitosan/apatite composite beads prepared by in situ generation of apatite or Siapatite nanocrystals. Acta Biomater., Vol. 6, 466–476.
  17. Murugan, R., & Ramakrishna, S. (2004). Bioresorbable composite bone paste using polysaccharide based nano hydroxyapatite. Biomaterials, Vol. 25, 3829–3835.
  18. Redepenning, J., Venkataraman, G., Chen, J., & Stafford, N. (2003). Electrochemical preparation of chitosan/hydroxyapatite composite coatings on titanium substrates. J. Biomed. Mater. Res., Vol. 66, 411–416.
  19. Pang, X., & Zhitomirsky, I. (2005). Electrodeposition of composite hydroxyapatite–chitosan films. Mater. Chem. Phys., Vol. 94, 245–251.
  20. Huang, Z., Dong, Y., Chu, C., & Lin P. (2008). Electrochemistry assisted reacting deposition of hydroxyapatite in porous chitosan scaffolds. Mater. Lett., Vol. 62, 3376–3378.
  21. Pang, X., Casagrande, T., & Zhitomirsky, I. (2009). Electrophoretic deposition of hydroxyapatite-CaSiO3 -chitosan composite coatings. J. Colloid Interface Sci., Vol. 330, 323–329.
  22. Pang, X., & Zhitomirsky, I. (2007). Electrophoretic deposition of composite hydroxyapatite-chitosan coatings. Mater. Charact., Vol. 58, 339–348.
  23. Sukhodub, L. B., Moseke, C., et al. (2003). Improved thermal substrate method with cooling system for hydroxyapatite coatings on titanium substrates. Annual Report. Institut für Kernphysik. Westfalishe Wilhelmsuniversität Münster, pp. 86–88.
  24. Yanovska, A., Kuznetsov, V., et al. (2011). Synthesis and characterization of hydroxyapatite-based coatings for medical implants obtained on chemically modified Ti6Al4V substrates. J. Surf. Coat. Technol., Vol. 205, 5324-5329.
  25. Horkavcová, D., Plešingerová, B., et al. (2008). Adhesion of the bioactive layer on titanium alloy substrate by Tape-test. Ceramics. Silikáty, Vol. 52, No. 3, 130–138.
  26. Viala, S., Freche, M., & Lacout J. L. (1998). Preparation of a new organic-mineral composite: chitosan-hydroxyapatite. Ann. Chim. Sci. Mater., Vol. 23, Issues 1–2, 69–72.

Full Text

Научный журнал "Журнал инженерных наук"
ISSN 2414-9381 (Online), ISSN 2312-2498 (Print).

Факультет технических систем и энергоэффективных технологий
Сумского государственного университета