Biosynthesis of Silver Nanoparticles Extracted Using Proteus | Journal of Engineering Sciences

Biosynthesis of Silver Nanoparticles Extracted Using Proteus

Author(s): Shameran J. S.1*, Sewgil S. A.2, Awara Kh. S.3

1 Department of Chemistry, Koya University, D. Mitterrand Blvd., Koya, Iraq;
2 Hawler Medical University, 100 M St.; PO Box 178, Erbil, Iraq;
Department of Biology, Koya University, D. Mitterrand Blvd., Koya, Iraq

*Corresponding Author’s Address:

Issue: Volume 6; Issue 2 (2019)

Paper received: January 29, 2019
The final version of the paper received: April 7, 2019
Paper accepted online: April 12, 2019

Shameran, J. S., Sewgil, S. A., Awara, Kh. S. (2019). Biosynthesis of silver nanoparticles extracted using Proteus. Journal of Engineering Sciences, Vol. 6(2), pp. C1-C5.

DOI: 10.21272/jes.2019.6(2).c1

Research Area:  MANUFACTURING ENGINEERING: Materials Science

Abstract. This study is focused on the evaluation of dependable and eco-friendly methods for the synthesis of metal nanoparticles is a significant step in the area of application of nanotechnology. One of the alternatives to obtain this purpose is to use natural techniques such as biological approach. Here, we examine biosynthesis of metallic nanoparticles using extract Proteus sp. the metal nanoparticles were successfully synthesized via reduction of silver sulfate employed extracted cell of bacterium Proteus sp. Nevertheless, the extracellular acts as a reducing agent to convert silver ion from its aqueous solution and the synthetic were formed within 2 hrs. On the other hand, scanning electron microscopy (SEM) which describes the surface morphology of bio-reduction of Ag-nanoparticles demonstrated that the spherical nature occurred through the bio-synthesis process and the particles are mostly circular and irregular in shape, UV-visible exhibit a peak at 423 nm corresponding to the plasmon of silver nanoparticle and XRD pattern was taken and presented that all peaks were indexed by hexagonal wurtzite phase (PIXcel 1D). In spite of that, the band gap energy measured (2.93 eV) and suggested strong scattering of the X-ray in the crystalline phase. Finally, we concluded that this study offers the remarkable report that biological synthetic of metal nanoparticle is helpful to avoid the negative influence of physical and chemical process that is inappropriate for medical applications.

Keywords: bandgap, Proteus, bio-reduction, metallic nanoparticle.


  1. Pliatsuk, L. D., Chernysh, Y. Y., Ablieieva, I. Y., Kozii, I. S., Balintova, M., Matiash, Y. O. (2018). Sulfur utilization in the systems of biological wastewater denitrification. Journal of Engineering Sciences, Vol. 5(1), pp. H7–H15,
  2. Ghosh, S., Jagtap, S., More, P., Shete, U. J., Maheshwari, N. O., Rao, S. J., Pal, J. K. (2015). Dioscorea bulbifera mediated synthesis of novel Au core Ag shell nanoparticles with potent antibiofilm and antileishmanial activity. Journal of Nanomaterials, Vol. 16(1), pp. 161.
  3. Salih, S. J., Smail, A. K. (2016). Synthesis, characterization and evaluation of antibacterial efficacy of zinc oxide nanoparticles. Pharmaceutical and Biological Evaluations, Vol. 3(3), pp. 327–333.
  4. Saravanan, M., Barik, S. K., MubarakAli, D., Prakash, P., Pugazhendhi, A. (2018). Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria. Microbial Pathogenesis, Vol. 116, pp. 221–226.
  5. Sarsar, V., Selwal, M. K., Selwal, K. K. (2015). Biofabrication, characterization and antibacterial efficacy of extracellular silver nanoparticles using novel fungal strain of Penicillium atramentosum KM. Journal of Saudi Chemical Society, Vol. 19(6), pp. 682–688.
  6. Fariq, A., Khan, T., Yasmin, A. (2017). Microbial synthesis of nanoparticles and their potential applications in biomedicine. Journal of Applied Biomedicine, Vol. 15(4), pp. 241–248.
  7. Balashanmugam, P., Santhosh, S., Giyaullah, H., Balakumaran, M. D., Kalaichelvan, P. T. (2013). Mycosynthesis, characterization and antibacterial activity of silver nanoparticles from Microporusxanthopus: a macro mushroom. International Journal of Innovative Research in Science, Engineering and Technology, Vol. 2(11), pp. 1–9.
  8. Kalpana, D., Lee, Y. S. (2013). Synthesis and characterization of bactericidal silver nanoparticles using cultural filtrate of simulated microgravity grown Klebsiella pneumoniae. Enzyme and Microbial Technology, Vol. 52(3), pp. 151–156.
  9. Ali, D. M., Sasikala, M., Gunasekaran, M., Thajuddin, N. (2011). Biosynthesis and characterization of silver nanoparticles using marine cyanobacterium, Oscillatoria willei NTDM01. Digest Journal of Nanomaterials and Biostructures, Vol. 6(2), pp. 385–390.
  10. Vahabi, K., Mansoori, G. A., Karimi, S. (2011). Biosynthesis of silver nanoparticles by fungus Trichoderma reesei (a route for large-scale production of AgNPs). Insciences Jornal, Vol. 1(1), pp. 65–79.
  11. Hosseini, M. R., Sarvi, M. N. (2015). Recent achievements in the microbial synthesis of semiconductor metal sulfide nanoparticles. Materials Science in Semiconductor Processing, Vol. 40, pp. 293–301.
  12. Salih, S. J., Rashid, B. Z. (2015). Cranberry stem as an efficient adsorbent and eco-friendly for removal of toxic dyes from industrial wastewater. Physico Studies. International Journal of Pharmaceutical Chemistry, Vol. 5(6), pp. 207–217.
  13. Usman, A. R., Kuzyakov, Y., Lorenz, K., Stahr, K. (2006). Remediation of a soil contaminated with heavy metals by immobilizing compounds. Journal of Plant Nutrition and Soil Science, Vol. 169(2), pp. 205–212.
  14. Yanovska, H. O., Bolshanina, S. B., Kuznetsov, V. M. (2017). Formation of hydroxyapatite coatings with addition of chitosan from aqueous solutions by thermal substrate method. Journal of Engineering Sciences, Vol. 4(2), 2017.
  15. Salih, S. J., Anwer, S. S., Faraj, R. H. (2017). A biosorption of Mercury from wastewater using isolated Aspergillus sp. Modified 1, 10-Phenanthroline: Hill isotherm model. Science Journal of University of Zakho, Vol. 5(4), pp. 288–295.
  16. Ray, C. G., Ryan, K. J. (2004). Sherris Medical Microbiology: An Introduction to Infectious Diseases. McGraw-Hill.
  17. Mobley, H. L., Belas, R., Lockatell, V., Chippendale, G., Trifillis, A. L., Johnson, D. E., Warren, J. W. (1996). Construction of a flagellum-negative mutant of Proteus mirabilis: effect on internalization by human renal epithelial cells and virulence in a mouse model of ascending urinary tract infection. Infection and Immunity, Vol. 64(12), pp. 5332–5340.
  18. Jayaseelan, C., Rahuman, A. A., Kirthi, A. V., Marimuthu, S., Santhoshkumar, T., Bagavan, A., Rao, K. B. (2012). Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, Vol. 90, pp. 78–84.
  19. Singh, P., Kim, Y. J., Zhang, D., Yang, D. C. (2016). Biological synthesis of nanoparticles from plants and microorganisms. Trends in Biotechnology, Vol. 34(7), pp. 588–599.
  20. Hussain, , Singh, N. B., Singh, A., Singh, H., Singh, S. C. (2016). Green synthesis of nanoparticles and its potential application. Biotechnology Letters, Vol. 38(4), pp. 545–560.
  21. Agarwal, H., Kumar, S. V., Rajeshkumar, S. (2017). A review on green synthesis of zinc oxide nanoparticles – An eco-friendly approach. Resource-Efficient Technologies, Vol. 3(4), pp. 406–413.
  22. Moghaddam, A., Namvar, F., Moniri, M., Azizi, S., Mohamad, R. (2015). Nanoparticles biosynthesized by fungi and yeast: a review of their preparation, properties, and medical applications. Molecules, Vol. 20(9), pp. 16540–16565.

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