Application of Technological Solutions for Bioremediation of Soils Contaminated with Heavy Metals | Journal of Engineering Sciences

Application of Technological Solutions for Bioremediation of Soils Contaminated with Heavy Metals

Author(s): Chernysh Y.1,2*, Plyatsuk L.1,2, Roubik H.2,3, Yakhnenko O.1, Skvortsova P.1, Bataltsev Y.1

1 Sumy State University, 2, Rymskogo-Korsakova St., 40007 Sumy, Ukraine;
2 International Innovation and Applied Center “Aquatic Artery”, 2, Rymskogo-Korsakova St., Sumy, 40007, Ukraine;
3 Czech University of Life Sciences Prague, Faculty of Tropical AgriSciences, 129, Kamycka St., 165 00 Prague, Czech Republic

*Corresponding Author’s Address: [email protected]

Issue: Volume 8, Issue 2 (2021)

Submitted: September 2, 2021
Accepted for publication: November 24, 2021
Available online: November 29, 2021

Chernysh Y., Plyatsuk L., Roubik H., Yakhnenko O., Skvortsova P., Bataltsev Y. (2021). Application of technological solutions for bioremediation of soils contaminated with heavy metals. Journal of Engineering Sciences, Vol. 8(2), pp. H8-H16, doi: 10.21272/jes.2021.8(2).h2

DOI: 10.21272/jes.2021.8(2).h2

Research Area:  CHEMICAL ENGINEERING: Environmental Protection

Abstract. This article focuses on studying biotechnologies for remediation of soils contaminated with heavy metals to determine further the most effective methods for cleaning soils from the action of toxicants with their subsequent implementation in practice. The soil restoration methods were analyzed, their advantages and disadvantages were identified, making it possible to establish that biological methods are the safest and most environmentally friendly. The expediency of using biological methods lies in the possibility of breeding strains of microorganisms that destroy soil toxicants. However, the efficiency of microbial cultures is not equally high due to a narrow range of favorable conditions for functioning, the risk of manifestation of the phenomenon of degeneration of microorganisms until the required level of soil purification is achieved. This confirms the prospects for the further development of this direction and the search for ways to eliminate certain disadvantages of biological methods. For an integrated biotechnological solution to soil remediation, a scheme of aerobic plants was developed, which is characterized by two stages: aerobic soil cultivation with biocomposite and a phytoremediation stage for additional purification and control of the content of toxicants in the soil.

Keywords: soil contamination, biotechnologies, remediation, heavy metals, biocomposite.


  1. Intergovernmental Forum on Chemical Safety Global Partnerships for Chemical Safety Contributing to the 2020 Goal. (2008). Review of the problem of environmental pollution with cadmium, lead and mercury in Russia and Ukraine. Retrieved from
  2. Zhang, J., Cao, X., Yao, Z., Lin, Q., Yan, B., Cui, X., He, Z., Yang, X., Wang, C.-H., Chen, G. (2021). Phytoremediation of Cd-contaminated farmland soil via various Sedum alfredii-oilseed rape cropping systems: Efficiency comparison and cost-benefit analysis. Journal of Hazardous Materials, Vol. 419, 126489, doi:
  3. Oladoye, P. O., Olowe, O. M., Asemoloye, M. D. (2021). Phytoremediation technology and food security impacts of heavy metal contaminated soils: A review. Chemosphere, 132555, doi:
  4. Chernysh, Y. Y., Plyatsuk, L. D. (2018). Binding of heavy metals in the soil complex at the introduction biocomposite based on sewage sludge and phosphogypsum. Young Scientist, Vol. 1 (53). Retrieved from
  5. Bathak, P., Bhattacharya, D. (2021). Phytoextraction of heavy metals by weeds: Physiological and molecular intervention. Handbook of Bioremediation. Physiological, Molecular and Biotechnological Interventions, pp. 49–59, doi:
  6. Nedjimi, B. (2021). Phytoremediation: a sustainable environmental technology for heavy metals decontamination. SN Applied Sciences, Vol. 3, 286, doi:
  7. Biodegradation of hydrocarbons as a solution to the problem of oil pollution. Retrieved from
  8. Nikovska, H. M., Ulberh, Z. R. (2003). Method for removal of heavy metal and radionuclids from soil. Patent of Ukraine, No. 58557.
  9. Xinhui, D., Ping, T., Mengqi, M., Liang, M., Yunjun, Y., Zhenxing, W. (2021). The Study of Conditions for Bioleaching Heavy Metals from Polluted Soil and Metabonomics in Penicillium chrysogenum. Journal of Biobased Materials and Bioenergy, Vol. 15(1), pp. 117–124(8), doi:
  10.  Nguyen, T. H., Won, S., Ha, M.-G., Nguyen, D. D., Kang, H. Y. (2021). Bioleaching for environmental remediation of toxic metals and metalloids: A review on soils, sediments, and mine tailings. Chemosphere, Vol. 282, 131108, doi:
  11.  Shtyka, O. S. Assessment of alternative remediation technologies of soils polluted with heavy metals. Retrieved from
  12. Shweta,N., Samatha, S., Keshavkant, S. (2021). Mechanisms, types, effectors, and methods of bioremediation: The universal solution. Microbial Ecology of Wastewater Treatment Plants, pp. 41–72, doi:
  13.  Raffa, C. M., Chiampo, F., Shanthakumar, S. (2021). Remediation of Metal/Metalloid-Polluted Soils: A Short Review. Applied Sciences, Vol. 11(9), doi:
  14.  Akbari, S., Abdurahman, N. H., Yunus, R. M., Fayaz, F., Alara, O. R. (2018). Biosurfactants – a new frontier for social and environmental safety: A review. Biotechnology Research and Innovation, Vol. 2(1), pp. 81–90, doi:
  15. Violante, A., Caporale, A. G. (2015). Biogeochemical processes at soil-root interface. Journal of soil science and plant nutrition, Vol. 15(2), pp. 422–448, doi:
  16.  Dobrovolsky, V. V. (2004). The role of soil organic matter in the migration of heavy metals. Nature, Vol. 7, pp. 35–39. Retrieved from
  17.  Adejumo S.A., Togun A.O., Adediran J.A., Ogundiran M.B. (2011) Field Assessment of Progressive Remediation of Soil Contaminated with Lead-Acid Battery Waste in Response to Compost Application. Pedologist, pp. 182–193.
  18.  Balseiro-Romero, M., Monterroso, C., Kidd, P. S., Lu-Chau, T. A., Gkorezis, P., Vangronsveld, J., Casares, J. J. (2018). Modelling the ex situ bioremediation of diesel-contaminated soil in a slurry bioreactor using a hydrocarbon-degrading inoculant. Journal of Environmental Management, Vol. 246, pp. 840–848, doi:
  19.  Han, Zuozhen, Dan Li, Hui Zhao, Huaxiao Yan, and Peiyuan Li. (2017). Precipitation of Carbonate Minerals Induced by the Halophilic Chromohalobacter Israelensis under High Salt Concentrations: Implications for Natural Environments. Minerals, Vol. 7(6), 95,
  20.  Li, J., Yang, H., Tong, L., Sand, W. (2021). Some Aspects of Industrial Heap Bioleaching Technology: From Basics to Practice. Mineral Processing and Extractive Metallurgy Review, doi:
  21.  Yanin, E. P. (2014). Remediation of territories contaminated with chemical elements: general approaches, legal aspects, main methods (Foreign experience). Environmental and natural resource issues, Vol. 3, pp. 3–105. Retrieved from
  22.  Rosestolato, D., Bagatin, R., Ferro, S. (2015). Electrokinetic remediation of soils polluted by heavy metals (mercury in particular). Chemical Engineering Journalthis, Vol. 264, pp. 16–23, doi:
  23.  Hazrati, S., Farahbakhsh, M., Cerdà, A., Heydarpoor, G. (2021). Functionalization of ultrasound enhanced sewage sludge-derived biochar: Physicochemical improvement and its effects on soil enzyme activities and heavy metals availability. Chemosphere, Vol. 269, 128767, doi:
  24.  Cameselle, C., Gouvei, S., Akretche, D. E., Belhadj, B. (2013). Advances in Electrokinetic Remediation for the Removal of Organic Contaminants in Soils. Organic Pollutants – Monitoring, Risk and Treatment, M. Nageeb Rashed, IntechOpen, doi: 10.5772/54334.
  25.  Jankaitë, A., Vasarevièius, S. (2005). Remediation technologies for soils contaminated with heavy metals. Journal of Environmental Engineering and Landscape Management, Vol. 13(2), pp. 109–113, doi: 10.3846/16486897.2005.9636854.
  26.  Guidelines on remediation of contaminated sites. Retrieved from
  27.  Chernysh Y., Ablieieva I., Makarekno N., Plyatsuk L., Trunova I., Burla O. (2021) Investigation of the directions of using a hybrid composition bioproduct for detoxification of a soil ecosystem contaminated with heavy metals and oil products. Biodiversity & Environment, Vol. 13(1), pp. 80–94.

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